Mutations are introduced into rearranged Ig variable genes at a frequency of 10?2 mutations per base pair by an unknown mechanism. amplified for another 30 rounds using nested primers with restriction sites for cloning the 466-bp product into M13 bacteriophage. The error rate for polymerase is 1.3 10?6 mutations per nucleotide per duplication, which would… Continue reading Mutations are introduced into rearranged Ig variable genes at a frequency